Česká společnost pro hmotnostní spektrometrii

(Česká konference hmotnostní spektrometrie 2023 - PL-3)
Introduction to three dimensional cryo- electron microscopy

Adam Schröfel ^1 *

1. Faculty of Science, Charles University, Prague

Abstrakt

This talk provides a concise introduction to three-dimensional cryo electron microscopy (3D cryo-EM) and electron tomography. We will cover the basic principles of sample preparation, imaging, and data collection using cryo-EM techniques, as well as the reconstruction methods involved in deriving high-resolution structures. Additionally, we will explore the application of electron tomography, a powerful extension of cryo-EM, for studying three-dimensional cellular architectures and larger macromolecular complexes. The talk aims to provide participants with a foundational understanding of 3D cryo-EM and electron tomography, showcasing their potential for advancing structural biology and uncovering intricate cellular details.

* Korespondující autor: adam.schrofel@natur.cuni.cz