CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - FrP-30)
Development of a novel approach for apocrine sweat microsampling and its utilization in breast cancer screening using pseudotargeted lipidomics

Aleš Kvasnička 1 *, Alena Tichá 2, Radomír Hyšpler 2, Hana Janecková 3, Radana Brumarová 1, Lukáš Najdekr 1, Zdenek Zadák 4, David Friedecký 3,1

  1. Faculty of Medicine and Dentistry, Palacký University Olomouc, Czechia
  2. Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czehcia
  3. Laboratory for Inherited Metabolic Disorders, University Hospital Olomouc, Czechia
  4. Department of Research and Development, University Hospital Hradec Králové, Czechia

Abstrakt

The intensive development of new non-invasive microsampling technologies is paving the way for a new era of patient friendly precision medicine. Since apocrine sweat (AS) has not yet been tested for this purpose so far, our aim was to look at the possibilities of its microsampling. We have designed a concept of 3D-printed attachment with porous glass filter disks called SLIDE (Sweat sampLIng DevicE) for easy sampling of AS. By applying pseudotargeted LC(C8)-MS(MRM) lipidomics the relevant lipids present in AS have been selected and semiquantified to evaluate the reproducibility and robustness of this novel approach. SLIDE was tested on 10 healthy individuals for three time points over a week, with mean variabilities for the lipid classes detected ranging from 26-64% and 51-119% for intraindividual and group variability (CVg), respectively. Probabilistic quotient normalization improved the CVg. The lipidomic content of AS was described in terms of identification and quantitation. A total of 240 lipids across 15 classes were identified. The lipid concentrations varied from 10-10 to 10-4 mol/L. The most numerous class of lipids was ceramides (Cer), while the free fatty acids (FFA) were the most abundant (10-5 mol/L on average). The applicability of SLIDE for breast cancer screening was tested on 20 clinically defined patients and 23 controls in parallel with the standard screening process in the mammology clinic. Patients were found to have increased concentrations of Cer and FFA and conversely decreased hexosylceramides. The main advantages of AS microsampling include the non-invasiveness of the procedure, speed of collection and patient comfort, while the application of SLIDE as an AS sampling technique brings new perspectives for use in modern clinical practice.

* Korespondující autor: kvasnicka.ales1@gmail.com

Literatura

  1. Kvasnička, A.; Friedecký, D.; Tichá, A.; Hyšpler, R.; Janečková, H.; Brumarová, R.; Najdekr, L.; Zadák, Z. SLIDE—Novel Approach to Apocrine Sweat Sampling for Lipid Profiling in Healthy Individuals. Int. J. Mol. Sci. 2021, 22, 8054. https://doi.org/10.339
  2. Sato, K.; Sato, F. Sweat secretion by human axillary apoeccrine sweat gland in vitro. Am. J. Physiol. 1987, 252, R181–R187.
  3. Baker, L.B. Physiology of sweat gland function: The roles of sweating and sweat composition in human health. Temperature (Austin) 2019, 6, 211–259.

Poděkování:

Funded by AZV CR NU20-08-00367.


Partneři společnosti

LabRules LCMS LabRules GCMS

Partneři

Bruker HPST Merck Pragolab Amedis EastPort Shimadzu Waters