CMSC - Presentation Abstract
Introduction to three dimensional cryo- electron microscopy
Adam Schröfel 1 *
- Faculty of Science, Charles University, Prague
Abstract
This talk provides a concise introduction to three-dimensional cryo electron microscopy (3D cryo-EM) and electron tomography. We will cover the basic principles of sample preparation, imaging, and data collection using cryo-EM techniques, as well as the reconstruction methods involved in deriving high-resolution structures. Additionally, we will explore the application of electron tomography, a powerful extension of cryo-EM, for studying three-dimensional cellular architectures and larger macromolecular complexes. The talk aims to provide participants with a foundational understanding of 3D cryo-EM and electron tomography, showcasing their potential for advancing structural biology and uncovering intricate cellular details.
* Corresponding author: adam.schrofel@natur.cuni.cz
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