CZechMS.org - Program - 13th Conference of Czech Society for Mass Spectrometry together with 11th Informal proteomic meeting (joint meeting)

Program - 13th Conference of Czech Society for Mass Spectrometry together with 11th Informal proteomic meeting (joint meeting)

Wednesday November 19, 2025

10:00 - 18:00 Registration
12:45 - 13:45 Lunch – Canteen, Biology Centre CAS
13:45 - 14:00 Conference Opening
14:00 - 15:00 Plenary Lecture I: Prof. Alexander Makarov (Thermo Fisher Scientific) (Chairperson: Zdeněk Kukačka)
PL-01: Past, present and future of Orbitrap Mass Spectrometry
15:00 - 16:00 Session I (Chairperson: Michael Volný)
- 15:00 - 15:20 Elliott James Price
  WeO-01: Towards MS-guided Precision Environmental Health: the Czech node of the European Research Infrastructure for Human Exposome Research
- 15:20 - 15:40 Urban Jiří
  WeO-02: Optimization of microflow LC-MS method by design of experiments and its application to proteomics analysis
- 15:40 - 16:00 Starovoit Mykyta
  WeO-03: Simultaneous mapping of peptides and released N-glycans by HILIC-FLD-MS for quality control of protein biopharmaceuticals
16:00 - 16:30 Coffee Break
16:30 - 16:50 Sponsor Lecture – Phenomenex
Lucia Geis-Asteggiante: Advancing Nano/Micro LC Proteomics with Core-Shell Technology
16:50 - 18:10 Session II (Chairperson: Jana Havlíková)
- 16:50 - 17:10 Melikov Aleksandr
  WeO-04: G-quadruplex – ligand interaction biophysics explored by native mass spectrometry and trapped ion mobility spectrometry
- 17:10 - 17:30 Smrčka Tomáš
  WeO-05: Insights into CyRepA2 primase-helicase-DNA interactions using structural mass spectrometry
- 17:30 - 17:50 Hájek Jan
  WeO-06: Breaking the Unbreakable: Structural Elucidation of the Hypermodified RiPP Nostatin A by IRMPD Mass Spectrometry
- 17:50 - 18:10 Portašiková Jasmína
  WeO-07: Unraveling Oligomerization-Mediated Inhibition in GGPPS by Structural Mass Spectrometry
18:10 - 18:30 Sponsor Lecture – Genetica
: A Multi-Approach Exploration of the Proteome: Integrating Three Platforms
18:30 - 19:00 Flash Talks I (Chairperson: Zdeněk Spáčil)
- Hebesberger Philipp: Quantification of Oxoacids of the Human Metabolome using Liquid Chromatography-Mass Spectrometry (WeS-01)
- Škeříková Veronika: Analyses of human scent samples (WeS-02)
- Grepl Jakub: rDUVLAESCI-MS/MSI: a novel approach for direct analysis of biological surfaces (WeS-03)
- Jágr Michal: Investigation of phenolic compounds in grape pomace through UHPLC-ESI-MS/MS (WeS-04)
- Brigante Federico: Mass Spectral Library Network (MERLIN) To Streamline The Generation Of Public Spectral Libraries (WeS-05)
- Mazumdar Aninda: A Comprehensive timsTOF MS/MS Spectral Library for Improving Metabolite Annotation in Non-Targeted Metabolomics (WeS-06)
19:00 - 22:00 Welcome Reception & Dinner (Canteen, live music from 19:30)

Thursday November 20, 2025

09:00 - 09:40 Workshop Pragolab
09:40 - 10:40 Session III (Chairperson: Tomáš Oždian)
- 09:40 - 10:00 Talacko Pavel
  ThO-08: Low-input proteomics using the Orbitrap Astral
- 10:00 - 10:20 Bouchal Pavel
  ThO-09: Integrated multiomics classification of triple negative breast cancer
- 10:20 - 10:40 Konik Peter
  ThO-10: Proteomics of human breast milk
10:40 - 11:00 Sponsor Lecture – Amedis
Tomáš Korba: SCIEX ZenoTOF 8600 – Sensitivity, Speed, Flexibility, Robustness
11:00 - 11:30 Coffee Break
11:30 - 12:10 Workshop Genetica
Barry Ewen: Quantum-Si: Advancing Proteomics: an introduction to NGPS
12:10 - 13:10 Session IV (Chairperson: Marek Vrbacký)
- 12:10 - 12:30 Černý Martin
  ThO-11: Development-dependent molecular strategies of stress adaptation in plants
- 12:30 - 12:50 Danchenko Maksym
  ThO-12: Deciphering adaptive shifts in the redox proteome of wheat seedlings under drought
- 12:50 - 13:10 Lochmanová Gabriela
  ThO-13: No Mark, No Problem: Alternative Epigenetic Silencing in Algae
13:10 - 14:00 Lunch
14:00 - 14:50 Plenary lecture II: Prof. Bernhard Küster (Technical University of Munich) (Chairperson: Juraj Lenčo)
PL-02: Proteomics advancing pharmacology
14:50 - 15:40 Flash Talks II (Chairperson: Petr Novák)
- Lenčo Juraj: Propionic acid boosts sensitivity in high-flow RPLC-MS proteomics compared to formic and acetic acid (ThS-07)
- Oždian Tomáš: Towards Biomarker Discovery: Multiomics analysis of Endometriosis (ThS-08)
- Šimoník Jan: Integrated proteogenomic analysis identifies synuclein gamma as a key target of the mesenchymal stem-like subtype of triple negative breast cancer (ThS-09)
- Vít Ondřej: Human IMPs: A tool for comparing outputs of different tools for transmembrane segment predictions in the human proteome (ThS-10)
- Lapčík Petr: LC-MS/MS-based terminomics unravels role of carboxypeptidase B1 in luminal A breast cancer (ThS-11)
- Sysel Jakub: Mass spectometry in hands of experimental biology: Impaired nucleotide salvage affects de novo synthesis in Drosophila melanogaster (ThS-12)
- Hinterholzová Helena: Bioaccumulation of pharmaceuticals in herbivorous fish: The role of aquatic plants as a dietary source (ThS-13)
- Koudelka Štěpán: LC-HRMS for large-scale chemical exposure studies (ThS-14)
15:40 - 17:00 Poster session + Coffee Break
17:00 - 17:40 Workshop Bruker
Gary Kruppa: Recent developments in proteomics instrument at Bruker: timsTOF AIP and timsOmni for ultra high sensitivity proteomics, PTM analysis and structural proteomics
Daniel Vláčil: timsMetabo™ Breakthrough 4D-Metabolomics and 4D-Lipidomics Sensitivity, Specificity and Annotation Confidence - at Speed, Depth, and Scale
17:40 - 18:00 Award Lecture – Josef Chmelík Prize Laureate (Chairperson: Pavel Bouchal)
18:00 - 18:20 General Assembly of the Czech Society for Mass Spectrometry (in Czech, lecture room)
18:00 - 18:20 Informal meeting of Proteomic section of CSBMB (in Czech or English, poster room)
19:00 - 22:00 Gala Dinner – Restaurant Solnice
21:00 Optional guided Walk-through historic center of České Budějovice – start at restaurant Solnice

Friday November 21, 2025

09:00 - 09:50 Plenary Lecture III: Prof. Jana Roithová (Radboud University) (Chairperson: Anton Škríba)
PL-03: Mass Spectrometry access to quantitative solution kinetics
09:50 - 10:50 Session V (Chairperson: Kristýna Gloc-Pimková)
- 09:50 - 10:10 Grabicová Kateřina
  FrO-15: Per- and polyfluorinated compounds (PFAS) in wild common bream
- 10:10 - 10:30 Kučera Lukáš
  FrO-16: Spatial mapping of 15N-labeled ammonia with MALDI-FTICR imaging in regenerating liver tissue: Re-directing of toxic byproduct into anabolic pathways
- 10:30 - 10:50 Čechová Aneta
  FrO-17: LC-ICP-MS analysis of anti-obesity peptides and their metabolites labeled with lanthanide mass-tag
10:50 - 11:00 Sponsor Lecture – Shimadzu
David Maxa: Advancing Structural Elucidation with Oxygen Attachment Dissociation (OAD)
11:00 - 11:20 Coffee Break
11:20 - 11:40 Sponsor Lecture – Bruker
Gary Kruppa: Emerging applications of bottom up proteomics in clinical research
11:40 - 13:00 Session VI (Chairperson: Volodymyr Pauk)
- 11:40 - 12:00 Stefani Tommaso
  FrO-18: Advancing Gut Microbiome Metabolomics: A Derivatization-Based Approach
- 12:00 - 12:20 Chalupský Karel
  FrO-19: Mass Spectrometry in Drug Development: From Screening to Stability and Pharmacokinetics
- 12:20 - 12:40 Kyjaková Pavlína
  FrO-20: Mass spectrometry of human body odor: Signatures of psychophysiological states
- 12:40 - 13:00 Coufalíková Kateřina
  FrO-21: Population-scale human chemical exposomics by automated liquid-liquid extraction coupled to gas chromatography – Orbitrap mass spectrometry
13:00 - 13:10 Closing Remarks and Awards
13:10 - 14:00 Lunch
14:00 - 17:40 Guided Tour – Samson Brewery / Temelín Nuclear Power Plant

List of posters:

WeS-01: Philipp Hebesberger: Quantification of Oxoacids of the Human Metabolome using Liquid Chromatography-Mass Spectrometry
WeS-02: Veronika Škeříková: Analyses of human scent samples
WeS-03: Jakub Grepl: rDUVLAESCI-MS/MSI: a novel approach for direct analysis of biological surfaces
WeS-04: Michal Jágr: INVESTIGATION OF PHENOLIC COMPOUNDS IN GRAPE POMACE THROUGH UHPLC-ESI-MS/MS
WeS-05: Federico Brigante: Mass Spectral Library Network (MERLIN) To Streamline The Generation Of Public Spectral Libraries
WeS-06: Aninda Mazumdar: A Comprehensive timsTOF MS/MS Spectral Library for Improving Metabolite Annotation in Non-Targeted Metabolomics
ThS-07: Juraj Lenčo: Propionic acid boosts sensitivity in high-flow RPLC-MS proteomics compared to formic and acetic acid
ThS-08: Tomáš Oždian: Towards Biomarker Discovery: Multiomics analysis of Endometriosis
ThS-09: Jan Šimoník: Integrated proteogenomic analysis identifies synuclein gamma as a key target of the mesenchymal stem-like subtype of triple negative breast cancer
ThS-10: Ondřej Vít: Human IMPs: A tool for comparing outputs of different tools for transmembrane segment predictions in the human proteome
ThS-11: Petr Lapcik: LC-MS/MS-based terminomics unravels role of carboxypeptidase B1 in luminal A breast cancer
ThS-12: Jakub Sysel: Mass spectometry in hands of experimental biology: Impaired nucleotide salvage affects de novo synthesis in Drosophila melanogaster
ThS-13: Helena Hinterholzová: Bioaccumulation of pharmaceuticals in herbivorous fish: The role of aquatic plants as a dietary source
ThS-14: Štěpán Koudelka: LC-HRMS for large-scale chemical exposure studies

ThP-01: Marek Vrbacky: Complexome profiling workflow elucidates mitochondrial cytochrome c oxidase biogenesis
ThP-02: Stanislav Opekar: Amino Acid Profiling Across Diverse Biological Matrices Using the MetAmino® LC-MS Kit: Applications in Free and Peptide-Bound Amino Acid Analysis
ThP-03: Alzbeta Nemeskalova: Ambient MS for rapid screening of pharmaceutically active compounds: Off-the-shelf and lab-made ion sources
ThP-04: Olha Lakhneko: ADAPTIVE PROTEOME UNDERPINS DROUGHT TOLERANCE IN BREAD WHEAT DURING FLOWERING
ThP-05: Volodymyr Pauk: Transition from natural dyestuffs to synthetic colorants depicted by ion mobility-aided mass spectrometry
ThP-06: Ulrika Malá: Off-line GC-GC×GC-MS focusing on scent samples
ThP-07: Adam Neuman: Ambient Soft Landing Voltammetry: Tool for Electrode Modification and Molecular Redox Characterization
ThP-08: Andrej Kovac: Translational research aimed at improving heatstroke diagnosis
ThP-09: Filip Gregar: Tracing the Gravettian Stone Paths
ThP-10: Petr Čičmanec: Leaf discs as a suitable alternative for cell cultures in plant omics analyses
ThP-11: Tomáš Pluháček: Characterization of Nanoparticles in Mixtures by Taylor Dispersion Analysis Hyphenated to Inductively Coupled Plasma Mass Spectrometry
ThP-12: Kateřina Kubátová: Sub-cellular interacting partners of the tick-borne encephalitis virus capsid protein
ThP-13: Jana Bellová: Mass Spectrometry-Based Proteomic Profiling of Sugiyamaella lignohabitans Adaptation to Xylose as Carbon Source
ThP-14: Jakub Červenka: Proteomic Analysis of Acetylsalicylic Acid-Induced Changes in the Cellular, Secreted, and Extracellular Vesicle Proteome of Malignant Melanocytes
ThP-15: Lada Fialová: Apparatus for Protein Footprinting by Singlet Oxygen
ThP-16: Filip Dyčka: Insight into Hemoglobin Digestion Pathways of Sphaerospora molnari by Functional Proteomics
ThP-17: Jana Knytlová: High-flow HESI interface with point-focusing and enhanced desolvation
ThP-18: Tereza Hrdinová: Proteomics identifies the landmark protein expression difference among the established mutation-based clusters of human pheochromocytoma and paraganglioma. Protein profiles can verify or correct diagnostic classification.
ThP-19: Mária Dobšovičová: Application of LC–MS/MS and fluorescence-based assays in the evaluation of novel carbonic anhydrase inhibitors
ThP-20: Jarmila Štěrbová: Production of sialylated glycoproteins in Ixodes ricinus ticks and tick cell lines
ThP-21: Jaroslav Galba: Application of LC–MS-Based “Polaromics” in Biochemistry, Pharmacology, and Biomedicine
ThP-22: Evgeniya Biryukova: Development of Software for LC-MS Data Processing of Oligonucleotides
ThP-23: Palina Ryzhaya: Enhanced Bottom-Up Proteomics for Histone Peptidoform Profiling
ThP-24: Daniel Smíšek: Detection of deoxynivalenol in grain products by LC-MS
ThP-25: Ivo Chamrád: FAmOS versus Percoll: Advancing Organelle Isolation Efficiency and Proteomic Compatibility in Plant Research
ThP-26: Jana Havlíková: Quantification of monoclonal antibodies by middle-up capillary electrophoresis coupled to mass spectrometry
ThP-27: Natálie Zemánková: Integrative proteomic and hormonomic analysis of barley chloroplast mutant chlorina f2f2
ThP-28: Petra Majerova: Microplastics and the Blood–Brain Barrier: Mechanisms of Transport and Endothelial Response
ThP-29: Marta Cruz: Reactivity of Singlet Oxygen Toward Amino Acid Side Chains
ThP-30: Alina Sadchenko: Applied and Residual: Assessment of Pesticide Residues and Transformation Products in Irrigated Czech Agricultural Fields.
ThP-31: Michael Karpíšek: Expanding the FFAP protocol for structural characterization of membrane proteins
ThP-32: Zuzana Kalaninová: DigDig: Streamlining Digestion Analysis for Proteomics and HDX-MS
ThP-33: Ezgi Gurler: Illuminating Proteins: Dual UV/IR Photodissociation in a custom FT-ICR system
ThP-34: Michaela Myšáková: Decoding Therapy-Induced Protein Subcellular Relocalization in Leukemia
ThP-35: Dmitry Loginov: Advancing AI/ML-Based Post-Translational Modification Assignment Using a Verified FPOP Dataset
ThP-36: Petr Zacek: Analysis of glycerophospholipids using the method of direct infusion into a tribrid mass spectrometer with high mass resolution
ThP-37: Chao Zhang: In situ comparison of hormonomics changes in different biotic stresses using mass spectrometry imaging
ThP-38: Katerina Kucerova: Large-scale metabolomics screening of understudied plant lineages
ThP-39: Dušan Nemes: Quantitative and Redox Proteomics Identify Signaling Networks Shaping Leukemia Survival and Resistance
ThP-40: Petr Halada: Deciphering phlebotomine sand fly taxonomy using MALDI-TOF MS protein profiling
ThP-41: Haya Alsulaiti: Metabolomics Analysis of Type 1 Diabetes Mellitus Among Qatari Children Using LC-MS