Česká společnost pro hmotnostní spektrometrii

(CEEPC/IPM/CMSC 2022 - ThP-43)
Development of A Method For Lipopeptide Separation Using Liposomes

Adéla Pravdová ^1,2 *, Martin Hubálek ¹

1. Ústav organické chemie a biochemie AV ČR
2. Katedra analytické chemie, Přírodovědecká fakulta, Univerzita Karlova

Abstrakt

Liposomes - vesicles formed by a phospholipid bilayer - are commonly used as a model of cell membranes in vitro experiments. The so-called Liposome-binding assay is a simple and inexpensive method how to prepare these vesicles and it has been used in many projects to investigate the interactions of whole proteins with the cell membrane (1,2). However, it is not known that liposomes have been used to test the interaction of membranes with lipopeptides, which are formed by cleavage of the entire lipoprotein and are directly responsible for binding to the phospholipid bilayer.
The aim of this project is to verify the functionality of the peptide-liposome system, as a possible way of separation of peptides with hydrophobic properties from a complex matrix, thus enable their qualitative and quantitative analysis, which is important for monitoring pathological processes, a differentiation or synaptic transmission in cells (3).
With modified Liposome-binding procedure, it was possible to isolate lipopeptides from mixture of peptides obtained by enzymatic digestion of Mouse mammary tumor virus matrix protein (MMTV). Almost 100% of the lipopeptides were separated and concentrated in the liposomal fraction.

* Korespondující autor: adela.pravdova@uochb.cas.cz

Literatura

1. Julkowska M. M. et al.: Methods Mol Biol. 1009, 261-71 (2013).
2. Junková P. et al.: J Biol Chem. 295(26), 8819-8833 (2020)
3. Resh M. D.: Prog Lipid Res. 63, 120-131 (2016)