Česká společnost pro hmotnostní spektrometrii

(13. ročník České konference hmotnostní spektrometrie a 11. Neformální proteomické setkání - ThS-12)
Mass spectometry in hands of experimental biology: Impaired nucleotide salvage affects de novo synthesis in Drosophila melanogaster

Jakub Sysel ¹, Tomáš Doležal ^1 *, Lenka Chodáková ¹, Martin Moos ²

1. University of South Bohemia
2. Biology Centre CAS

Abstrakt

Nucleotide metabolism is essential for maintaining cellular homeostasis, with synthesis pathways often impaired in disease. However, the metabolic compensation mechanisms that occur when key enzymes of the nucleotide salvage pathway are disrupted remain poorly understood. Here, we employed LC-MS combined with ¹³C labelled glucose to trace metabolite flux in Drosophila melanogaster larvae lacking ribokinase (RBKS), a key enzyme in nucleotide salvage. Our results show significantly reduced labelling of intermediate from the de novo synthesis pathway.

This methodology provides new insights into nucleotide metabolism and underscores the importance of the salvage pathway in maintaining nucleotide balance.

* Korespondující autor: kubasyslik@seznam.cz

Literatura

1. Robinson A. et al.: Cancer Letters 470, (2019)