CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - SaO-24)
Cervical mucus – a non-invasive uterine biomarker source?

Tomáš Oždian 1 *, Jiří Dostál 2, Dušan Holub 1, Rastislav Slavkovský 1, Jan Vodička 2, Michal Ješeta 3, Radova Pilka 2, Marián Hajdúch 1, Petr Džubák 1

  1. Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacký University in Olomouc
  2. Department of Gynecology and Obstetrics, University Hospital in Olomouc, Faculty of Medicine, Palacký University in Olomouc
  3. Center of Assisted Reproduction CAR 01 Brno, Obilní trh 11, Brno, Czech Republic

Abstrakt

Cervical mucus (CM) is a viscous fluid produced by cervical glands located in the myometrium of the uterine cervix. Usually, CM forms a plug between vagina and uterus. During the ovulation, CM starts to be more fluidic to allow the sperm to go through. During the ovulation, there is a good window for non-invasive sampling of the CM, originating from uterine derived tissues. The main hypothesis for CM proteomic study is to develop a method for CM sampling, processing and to answer if there are enough proteins in the CM for potential biomarker search. The resulting protocol consists of mucus dissolution, multi-enzyme digestion, and LC-MS proteomic analysis. The optimized approach allows to identify more than thousand proteins from every sample tested. The bioinformatic analysis reveals that the identified proteins are both intra and extra cellular and discovers the proteins with enriched expression in the broader female reproductive tract. To our best knowledge, we provide the most extensive proteomic characterization available.

* Korespondující autor: ozdiant@seznam.cz

Poděkování:

This work was supported by Czech Health Research Council (NV-18-02-00291), European Regional Development Fund - Project ENOCH (No. CZ.02.1.01/0.0/0.0/16_019/0000868) and EATRIS plus (871096).


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