Česká společnost pro hmotnostní spektrometrii

(CEEPC/IPM/CMSC 2022 - FrO-07)
Qualitative and quantitative advancements of QTOF performance: the SCIEX ZenoTOF 7600 system

Volker Kruft ^1 *

1. SCIEX

Abstrakt

A novel hybrid collision cell is at the heart of the technological innovations introduced with the SCIEX ZenoTOF 7600 system.
In the past, QTOF mass spectrometers have suffered from duty cycle losses; that is, losses in ion transmission in the ion path. This was mainly due to the mating the continuous beam coming from the quadrupole ion path with time-of-flight (TOF) analysis, a pulsed, discontinuous measurement technique. A series of ion-staging events and reverse-mass sequential ion release, with high-capacity ion traps, have been introduced just after the CID collision cell (Q2) and before the pusher region of the TOF. This allows the duty cycle losses to be mitigated leading to MS/MS sensitivity gains of 4-20 fold [1].
The newly engineered collision cell also has the ability to perform both collision induced dissociation (CID) and electron activated dissociation (EAD) experiments for high-resolution, high sensitivity MS/MS flexibility. Electron kinetic energies can be tuned from 0-25 eV without the use of chemical transfer reagents. This precise tunability means EAD can be performed on a wide range of analytes , from multiply charged peptides to singly-charged small molecules [2].

The increase in MS/MS sensitivity, the high speed of acquisition (133Hz) and the choice of fragmentation regimes enables improvements in data quality and depth on analysis in proteomics, metabolomics and lipidomics workflows. Protein IDs from cell lines exceeds 5000 protein groups with up to 95% of these reliably quantitated (CV <20%) at minimal protein loads. Lipids can be fully characterized at an LC time scale (around 30ms EAD reaction time) including their lipid class, acyl group structure, and the location of double bond(s) [3].

* Korespondující autor: volker.kruft@sciex.com

Literatura

1. Loboda A.V., Chernushevich I.V.: A novel ion trap that enables high duty cycle and wide m/z range on an orthogonal injection TOF mass spectrometer. J Am Soc Mass Spectrom.; 20: 1342-1348 (2009).
2. Baba T. et al.: Dissociation of Biomolecules by an Intense Low-Energy Electron Beam in a High Sensitivity Time-of-Flight Mass Spectrometer. J Am Soc Mass Spectrom. 32, 1964-1975 (2021).
3. Baba T. et al.: Distinguishing cis and trans isomers in intact complex lipids using electron impact excitation of ions from organics (EIEIO) mass spectrometry. Anal. Chem. 2017, 89: 7307–7315 (2017).