Česká společnost pro hmotnostní spektrometrii

(CEEPC/IPM/CMSC 2022 - ThP-39)
Potential of ASAP-MS Technique and its Application for Metabolite Profiling in Plant Microsamples

Petra Krejčí ^1 *, Jana Nádvorníková ¹, Zbyněk Žingor ¹, Štěpán Dostál ¹, Lucie Kobrlová ¹, Petr Bednář ¹

1. Univerzita Palackého v Olomouci

Abstrakt

In this communication we introduce a new atmospheric solids analysis probe mass spectrometric (ASAP-MS) technique for direct analysis of solid plant microsamples. Lab modified glass capillaries were used as probe in connection with a high-resolution tandem mass spectrometer Cyclic IMS (Waters). The sampling was performed using a defined micromanipulation under microscopic control.
Long chain fatty acids (LFA), their hydroxy-derivates (HLFA) and alkyl esters of caffeic acid (AECA) were detected and identified by this technique in intact microsamples of pea seed coat parts. Effect of external degradation factors (UV light, ozonation or heat) on metabolite profile was studied. Significant degradation of (H)LFA and AECA was observed. On the other hand, sterols (e.g. β-sitosterol, stigmasterol and campesterol) and phenolic compounds (sinapic acid, catechin etc.) were identified in all microsamples without significant changes. Moreover, new degradation products (e.g. a signal at m/z 413.3811) appeared in exposed microsamples and their identification is an objective of present research.
Combination of micromanipulation with microscopic control for sampling and ASAP-MS analysis using modified capillaries appeared to be appropriate approach for metabolite profiling in plant microsamples.

* Korespondující autor: petra.v.krejci@gmail.com

Literatura

1. Krejčí P. et al: Talanta 242 (2022).
2. Cechová M. et al: Anal. Bioanal. Chem. 411, 1169-1180 (2019).
3. Cechová M. et al.: Int. J. Mol. Sci. 18, 2196 (2017).

Poděkování:

OP RDE project DSGC-2021-0102, entitled “Multimodal analysis of plant metabolites and their degradation products in microsamples” (CZ.02.2.69/0.0/0.0/19_073/0016713) is gratefully acknowledged.