CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - FrP-38)
Targeted mass spectrometry to identify optimal culture conditions for neural differentiation

Rita Sucha 1, Jakub Cervenka 1,2, Martina Kubickova 1,2, Marian Hruska-Plochan 3, Dasa Bohaciakova 4, Katerina Vodickova Kepkova 1, Tereza Novakova 1, Katerina Budkova 1, Hana Kovarova 1, Petr Vodicka 1 *

  1. Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, CZ
  2. Department of Cell Biology, Faculty of Science, Charles University, Prague, CZ
  3. Department of Quantitative Biomedicine, University of Zurich, Zurich, CH
  4. Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, CZ

Abstrakt

In vitro-propagated neural stem cells require specific culture conditions to retain their multipotentiality or differentiate into adult neural cells. However, a defining signature of cells that differentiate upon defined conditions into desirable neuronal/glial phenotype remains insufficiently characterized. We applied SRM to measure levels of protein markers routinely used to probe neural differentiation in a panel of well-described conditions. Our method helped to identify the presence of pluripotent, multipotent as well as lineage-committed cells [1]. Since the capacity of the measurement allows to quantify differentiation markers together with other relevant proteins simultaneously, we aimed to develop assays for additional markers that would provide a system-wide view. We browsed manually curated databases and selected 360 proteins that report on specific biological processes on the basis of literature evidence. The proteins are mechanistically employed in biological processes of neural differentiation, including cell proliferation, autophagy, apoptosis, etc. Such an expanded assay could allow the optimization of novel specific propagation and differentiation protocols, further increasing the accuracy in detecting targeted cell populations.

* Korespondující autor: vodicka@iapg.cas.cz

Literatura

  1. Sucha R. et al.: Biol Open 10(8):bio058727 (2021).

Poděkování:

This research was supported by the Czech Science Foundation (22-24983S), the Operational Programme Research, Development and Education (CZ.02.1.01/0.0/0.0/16_019/0000785), and the Czech Ministry of Education, Youth and Sports project InterCOST (LTC18079) under CellFit COST Action (CA16119).


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