CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - ThP-37)
Development and validation of a high throughput CZE-MS/MS method for sensitive quantification of triptorelin in pharmaceutical and biological matrices

Ondrej Štefánik 1 *, Ivana Čižmárová 1, Michaela Matušková 1, Andrea Horniaková 1, Petra Majerová 2, Peter Mikuš 1,3, Juraj Piešťanský 1,3

  1. Katedra farmaceutickej analýzy a nukleárnej farmácie, FAF UK
  2. Neuroimunologický ústav SAV
  3. Toxikologické a antidopingové centrum, FAF UK

Abstrakt

Triptorelin is a modulator of the gonadotropin-releasing hormone receptor and is clinically used in the treatment of hormone-responsive breast cancers, prostate cancer, endometriosis, female infertility, precocious puberty, and in the treatment of male hypersexuality. Triptorelin can also be abused in order to increase growth and improve athletic performance [1]. The aim of the present study is to develop a method based on capillary zone electrophoresis-tandem mass spectrometry (triple quadrupole) with multisegment injection and electrokinetic injection of the sample in order to achieve higher throughput and improved values of LOD and LOQ in both water and plasma matrices. The separation step was performed on a background electrolyte composed of 1000 mM formic acid at pH 1.88. We also compared the LODs of hydrodynamic and electrokinetic injections of triptorelin (0.25 and 0.005 µg/mL). The electrokinetic injection preconcentration technique reduces the LOD value 50 times compared to hydrodynamic injection. The multisegment injection approach, on the other hand, increases sample throughput 3 times. A highly selective and reliable determination of triptorelin was carried out in the MRM mode, using two ion transitions: 656.5→328.3 and 656.5→249.0. The developed and optimized method was then characterized by favorable performance parameters, such as limit of detection (5 ng/mL in water matrix, 25 ng/mL in plasma matrix), precision (relative standard deviation, 1.5-9.4% for intraday and 2.3-11.9% for interday reproducibility), and accuracy (relative errors in the range of 80-109%). Satisfactory performance parameters predetermine the proposed method for routine use in drug quality control laboratories and even in therapeutic drug monitoring.

* Korespondující autor: stefanik38@uniba.sk

Literatura

  1. J.E. Frampton, Triptorelin: A Review of its Use as an Adjuvant Anticancer Therapy in Early Breast Cancer, Drugs. 77 (2017) 2037–2048. https://doi.org/10.1007/s40265-017-0849-3.

Poděkování:

This work was supported by the projects VEGA 1/0514/22, KEGA 027UK-4/2020, APVV-15-0585 and FaF/2/2022, and was carried out in the Toxicological and Antidoping Center at the Faculty of Pharmacy, Comenius University in Bratislava.


Partneři společnosti

LabRules LCMS LabRules GCMS

Partneři

Bruker HPST Merck Pragolab Amedis EastPort Shimadzu Waters