Česká společnost pro hmotnostní spektrometrii

(13. ročník České konference hmotnostní spektrometrie a 11. Neformální proteomické setkání - PL-01)
Past, present and future of Orbitrap Mass Spectrometry

Alexander Makarov ^1 *

1. Thermo Fisher Scientific GmbH

Abstrakt

Since its commercial debut two decades ago, the utility of the Orbitrap™ analyzer has been continuously extended by additional capabilities such as quantitative analysis, new fragmentation methods, diverse vacuum and ambient ion sources, imaging, ion mobility and unprecedented extension of mass range. These enhancements evolved over multiple major families of Orbitrap-based instruments that became a real workhorse of many applications.

As the latest example of such evolution, the asymmetric track lossless (Astral™) analyzer is combining together Orbitrap and time-of-flight features to dramatically improve sensitivity and throughput of proteomic analysis. Performance and operation of this newest analyzer are described in detail, especially for single-cell proteomic experiments.

An overview of main trends in evolution of mass spectrometry instrumentation demonstrates ample reserves in further expansion of analytical capabilities, mainly via combining advanced analyzers with improved ion scheduling and utilization.

The talk concludes with the belief that the latest rapid progress in all aspects of instrumentation and analysis will enable mass spectrometry to remain competitive against alternative technologies and to rise to the challenge of unprecedented throughput of very deep analyses of very complex samples.

* Korespondující autor: alexander.makarov@thermofisher.com