Česká společnost pro hmotnostní spektrometrii

(CEEPC/IPM/CMSC 2022 - PL-02)
An FT-ICR-MS metabolomic journey

Marta Sousa Silva ¹, António Ferreira ¹, João Luz ¹, Mariana Louro ¹, Rodrigo Osawa ¹, Francisco Traquete ¹, Henrique Silva ¹, Mónica Soeiro ¹, Mariana Pereira ¹, Carlos Cordeiro ^1 *

1. FT-ICR and Structural MS Laboratory, MARE – Environmental an Marine Research Centre, Faculdade de Ciências, Universidade de Lisboa, Portugal

Abstrakt

Untargeted metabolomics provides a broad survey of the instant composition of living cells or biological fluids. Dynamic range and chemical diversity of the metabolome are the main challenges for its meaningful in depth characterization. Extreme resolution and mass accuracy MS decoupled from chromatographic separation allow the simultaneous analysis of the most diverse range of metabolites in a short period of time, enabling high throughput studies. Here we show the application of FT-ICR-MS to several biological, environmental and forensic problems, highlight the role of computational methods to transform MS spectra into biological information.

* Korespondující autor: cacordeiro@fc.ul.pt