Česká společnost pro hmotnostní spektrometrii

(CEEPC/IPM/CMSC 2022 - FrP-34)
Authenticity of animal furs using proteomics

David Straka ^1 *

1. Vysoká škola chemicko-technologická v Praze

Abstrakt

In order to reveal counterfeit fur, to protect protected mammalian species and for other forensic purposes, it is necessary to correctly determine the animal origin of the fur. One of the possibilities is to use of the proteomic approach that preferably targets the keratin protein, which is species-specific and, in addition, is present in large quantities in animal fur. In this work, we tried to overcome the most common obstacles in proteomic analysis of animal fur, which includes e.g. the presence of surface-bound lipids or the problem of obtaining the main protein of our interest – keratin. The reference materials were fur samples of fox, mink, muskrat and rabbit. The effects of disintegration of fur, delipidation and protein extraction on the results obtained by mass spectrometry techniques, namely matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometry (LC-ESI-Q-TOF) are shown and evaluated. The potential species-specific peptide markers for both mass spectrometric methods were successfully found.

* Korespondující autor: strakad@vscht.cz