Česká společnost pro hmotnostní spektrometrii

(5. konference České společnosti pro hmotnostní spektrometrii - PL-1)
Development of a surface acoustic wave nebulization ion source

David Goodlett ^1 *

1. University of Maryland

Abstrakt

A surface acoustic wave (SAW) is an acoustic wave traveling along the surface of a material. This phenomenon has been studied extensively in the field of electronics where SAW devices are widely used as filters, oscillators and transformers [1]. In 2010 the first paper describing their use for ion production for mass spectrometry was published, and the phrase ‘surface acoustic wave nebulization’ (SAWN) coined to describe this phenomenon [2]. Ions produced by SAWN are done so either in a continuous mode from a syringe pump depositing liquid on a chip or in a pulsed manner. In either mode nebulization occurs from a planar surface, with the opportunity to detect either positive- or negative-ions. Notably, SAWN mass spectra of chemical compounds in solution, such as peptides and proteins that are chemically basic in nature, commonly exhibit a shift toward lower average charge state distribution than is observed by electrospray ionization (ESI) of the same solution. Importantly, given that no DC voltage is applied to the liquid sample to produce ions, the SAWN nebulized ions have been shown to produce ions of lower internal energy than ESI [3]. We’ll present the results of our latest efforts to develop this method and couple it to digital microfluidics, for sample preparation on chip, as well as to a novel, hybrid ion mobility spectrometer coupled to a QExactive Orbitrap. Finally, we’ll review some very simple applications that benefit from the simplicity and speed of SAWN like analysis of fermented beverages.

* Korespondující autor: dgoodlett@rx.umaryland.edu

Literatura

1. Lange K. et al.: Anal. Bioanal. Chem. 391(5), 1509-1519 (2008).
2. Heron S. R. et al.: Anal. Chem. 82(10), 3985-3989 (2010).
3. Huang Y. et al.: J. Am. Soc. Mass Spectrom. 23(6), 1062-70 (2012).