Česká společnost pro hmotnostní spektrometrii

(13. ročník České konference hmotnostní spektrometrie a 11. Neformální proteomické setkání - FrO-21)
Population-scale human chemical exposomics by automated liquid-liquid extraction coupled to gas chromatography – Orbitrap mass spectrometry

Kateřina Coufalíková ^1,2, Romana Ševčíková ^1,2, Moira Zanaboni ³, Manuela Bergna ³, Renzo Picenoni ⁴, Akrem Jbebli ¹, Elliott James Price ^1,2 *

1. RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic
2. Czech node of the European Infrastructure for Human Exposome Research (EIRENE-CZ), Brno, CZ
3. Thermo Fisher Scientific, Milan, Italy
4. CTC Analytics AG, Zwingen, Switzerland

Abstrakt

Profiling chemical exposure in large-scale human population studies is analytically challenging in terms of robustness and reproducibility, as well as laboriousness of the entire procedure. Few academic settings conduct routine analysis of studies comprising > 1000 samples. Manual sample preparation is time consuming, requires high solvent consumption, and contributes to sample-to-sample variation. Sequential on-line sample preparation offers a solution, but adoption is currently lacking in routine application.
Within the framework of ERIENE-CZ, we have developed a fully automated sample preparation workflow coupled to gas chromatography - Orbitrap mass spectrometry (GC-Orbitrap MS) for large-scale screening of chemical exposure agents in blood serum and plasma. A fully automated liquid-liquid extraction of blood (100 µL) has been developed using a cartesian autosampler coupled to GC-Orbitrap MS. In brief, analytes are separated on a Rxi-5Sil MS column with ~14 min temperature gradient (80 °C to 330 °C), electron ionisation at 70eV, and mass spectra recorded from 70-700 m/z at 60K resolving power. Quality assurance and quality control (QA/QC) includes the analysis of certified mixtures of alkanes and polychlorinated biphenyls, alongside the standard reference materials (SRM) NIST 1950, NIST 1957, NIST 1958 in each analytical batch.
We shall present i) a critical appraisal of the implemented method, including the interventional maintenance schedule and systematically recorded errors, such as vial drop rates; ii) updates on the status of multi-country, multi-site method standardisation and harmonisation; and iii) report on application to multiple cohort studies, each comprising > 1000 samples.

* Korespondující autor: elliott.james.price@gmail.com

Literatura

1. https://doi.org/10.5281/zenodo.10612856
2. https://doi.org/10.1038/s41467-021-25840-9

Poděkování:

The research has been conducted within the European Infrastructure for Human Exposome Research (EIRENE RI), in cooperation with the Network of Exposomics in the US (NEXUS) and within the collaborative framework of the International Human Exposome Network (IHEN; HEU programme grant agreement 101137317). The authors acknowledge the EU’s H2020 grant agreements 857560 (CETOCOEN Excellence), 874583 (ATHLETE) and 874627 (EXPANSE); the HEU grant agreements 101079789 (EIRENE PPP) and 101096888 (DISCERN); and the RECETOX Research Infrastructure (LM2023069) financed by the MEYS (CZ.02.1.01/0.0/0.0/17_043/0009632). The views expressed are those of the author(s) and do not necessarily reflect those of the EU or REA.