Česká společnost pro hmotnostní spektrometrii

(CEEPC/IPM/CMSC 2022 - FrO-14)
Mass spectrometric analysis of intact proteins: the dark side of deconvolution

Ágnes Gömöry ¹, Károly Vékey ¹, László Drahos ^1 *

1. Research Centre for Natural Sciences, Budapest, Hungary

Abstrakt

One of the most commonly used technique for the study of intact proteins, including monoclonal antibodies (MABs), is the HPLC-MS. Study of pure, large amounts of protein is fairly easy, but for mixtures the situation is more complicated. However, the real challenge is to study small amounts of protein mixtures having large molecular mass. In this case, automatic evaluation (e.g. deconvolution of mass spectra) can give incorrect results and HPLC separation of the mixture is becoming increasingly important.

In this presentation, I would like to illustrate the process, problems and difficulties of evaluation from simple proteins to the study of complex antibody-drug conjugates. In the latter case, when the mixture is too complex, a reliable result can only be obtained using a detailed manual evaluation of the ion chromatograms and mass spectra.

* Korespondující autor: drahos.laszlo@ttk.hu