CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - ThP-29)
Adipocyte and adipose tissue proteome signatures in obesity

Pavel Hruška 1,2 *, Jan Kučera 3, Matěj Pekař 4,5, Pavol Holeczy 4,6, Miloslav Mazur 4, Marek Bužga 7,8, Daniela Kuruczová 9, Peter Lenárt 3, Jana Fialová Kučerová 1, David Potěšil 2, Zbyněk Zdráhal 2, Julie Bienertová-Vašků 1,3

  1. Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
  2. Central European Institute of Technology, Masaryk University, Brno, Czech Republic
  3. Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Brno, Czech Republic
  4. Department of Surgery, Vitkovice Hospital, Ostrava, Czech Republic
  5. Department of Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
  6. Department of Surgical Disciplines, Faculty of Medicine, University of Ostrava, Czech Republic
  7. Department of Human Movement Studies, Faculty of Education, University of Ostrava, Czech Republic
  8. Department of Physiology and Pathophysiology, Faculty of Medicine, University of Ostrava, Czech Republic
  9. Incubator of Kinanthropology Research, Faculty of Sport Studies, Masaryk University, Brno, Czech Republic

Abstrakt

The adipose tissue (AT) distribution is a significant factor in obesity pathophysiology and obesity-associated comorbidities. However, its role in obesity is still poorly understood. The tissue heterogeneity introduces intrinsic characteristics making the interpretation of the expression studies intricate. Therefore, we performed untargeted proteomics analysis of subcutaneous and omental primary adipocytes, the major AT cell type, followed by proteomics profiling of the respective AT. In total, we identified 3686 proteins in the adipocyte’s proteome and 4506 proteins in the AT proteome. The differential expression analysis revealed 1140 and 1249 differentially expressed proteins in adipocytes and AT, respectively. Subsequent bioinformatics analyses highlight the most differentially expressed pathways and processes but also show tissue characteristics derived from adipocytes. Our analysis represents a valuable resource on protein expression in adipocytes and AT, highlighting the key features contributing to obesity pathophysiology.

* Korespondující autor: pavel.hruska@med.muni.cz


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