Česká společnost pro hmotnostní spektrometrii

(13. ročník České konference hmotnostní spektrometrie a 11. Neformální proteomické setkání - FrO-17)
LC-ICP-MS analysis of anti-obesity peptides and their metabolites labeled with lanthanide mass-tag

Aneta Čechová ^1,2 *, Antonín Káňa ¹, Tomáš David ², Miroslav Polášek ², Jaroslav Kuneš ², Lenka Maletínská ², David Sýkora ¹

1. University of Chemistry and Technology, Prague, Czech Republic
2. Institute of Organic Chemistry and Biochemistry, Prague, Czech Republic

Abstrakt

Neuropeptide prolactin-releasing peptide analogs (PrRP31) show promise for treating obesity and related diseases. Lipidization with fatty acids (palm¹¹-PrRP31) improves peptide stability and reduces food intake and body weight in rodents, but its mechanism remains unclear. To track palm¹¹-PrRP31 in vivo using the Wistar rats, we labeled it with a ClickZip lanthanide mass tag and quantified it using ICP-MS. The tag’s exceptional stability allowed precise detection in biological tissues¹. To properly identify the metabolites of the administered palm¹¹-PrRP31, we developed and validated
a reversed-phase LC-ICP-MS method, overcoming the incompatibility of organic solvents with
ICP-MS by using 1,2-hexanediole. The optimized method met the EURACHEM validation guidelines and enabled the reliable determination of ClickZip-tagged anti-obesity lipopeptide and its metabolites in vivo².

* Korespondující autor: aneta.myskova@outlook.com

Literatura

1. David, Tomáš; Šedinová, Miroslava; Myšková, Aneta, et al. Ultra-inert lanthanide chelates as mass tags for multiplexed bioanalysis. Nat. Comm. 15, (2024): 9836.
2. Myšková, Aneta, et al. "Liquid chromatography–inductively coupled plasma mass spectrometry analysis of peptides labelled with ClickZip mass tags." Anal. Chim. Acta (2025): 343853.