CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - FrP-26)
Assessment of the effect of pretreatment of birch bark by supercritical extraction (scCO2) with the following Soxhlet extraction: semiquantitative analysis of compounds by GC/MS

Marek Kalanin 1 *, Aleš Ház 1, Richard Nadányi 1, Anton Lisý 1

  1. Department of Wood, Pulp and Paper, UPSP FCHPT STU, Bratislava, Slovakia

Abstrakt

The bark of trees is an important part of biomass. Many researchers have highlighted the potential of different species of tree bark in terms of their valorization to obtain value-added substances. Considerable attention has also been paid to the processing of bark from various species of the Betulaceae family. Extraction techniques are focused on isolating triterpenoids. The aim of this study was to assess the impact of pretreatment of birch bark by supercritical fluid extraction (scCO2) with the following Soxhlet extraction, and semiquantitative analysis of extractive substances by GC/MS.

The method to obtain desired product was supercritical fluid extraction using carbon dioxide under various conditions, followed by Soxhlet extraction, where a mixture of acetone and petroleum ether was used as a solvent. Obtained extracts were analyzed by GC/MS. The separation of substances was carried out on a capillary column HP-5MS. The stationary phase consisted of (5%-phenyl)-methylpolysiloxane, and the carrier gas helium with a flow rate of 2 ml/min. The end of the capillary column was connected to MS with an electron ionizer and a quadrupole analyzer. The range of measured masses (m/z) was 50 – 600 amu. The substances found in the extracts were identified according to the National Institute of Standards and Technology mass spectra library.

The analysis of extracts confirmed the presence of triterpenoids lupeol, betulin, bergamotene, and friedelan-3-one and their derivatives. The semiquantitative analysis showed the most promising results for lupeol with yields up to 61%.

* Korespondující autor: xkalaninm@stuba.sk

Poděkování:

This publication was supported by the Operational Program Integrated Infrastructure for the project: “Strategic research in the field of SMART monitoring, treatment, and preventive protection against coronavirus (SARS-CoV-2),” Project no. 313011ASS8, co-financed by the European Regional Development Fund (ERDF).


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