CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - ThP-25)
Using MALDI-TOF MS for the detection of putative new Acinetobacter species from cattle feces

Violetta Shestivska 1 *, Martina Maixnerová 2, Eva Skřivanová 3, Eva Vlková 3, Martina Kyselková 4, Alexandr Nemec 2

  1. National Institute of Public Health Prague, CZECH REPUBLIC
  2. National Institute of Public Health Prague
  3. Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague
  4. Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS Prague


Question: Acinetobacter is a taxonomically diverse bacterial genus, with 72 validly named species and many provisional taxa [1]. The genus is ubiquitous, inhabiting plethora of natural ecosystems. Acinetobacter spp., with A. baumannii in particular, have emerged as opportunistic human pathogens, associated with multidrug resistance and epidemic spread [2]. As the knowledge on Acinetobacters in non-human ecosystems is limited, we conducted a study on the impact of antibiotic use in livestock on the occurrence and spread of resistant and potentially pathogenic Acinetobacter spp. in cattle feces.

Methods: Samples of cattle feces were homogenized and cultured aerobically in a mineral medium supplemented with sodium acetate. The grown-up cultures were streaked onto both acetate agar and chrome agar plates. Up to 24 agar-grown colonies per sample were directly identified by MALDI-TOF MS using the current Bruker database supplemented with homemade entries of provisional Acinetobacter taxa. The spectra were further compared using cluster analysis to dereplicate isolates of the same strains.

Results: A total of 19 samples from 16 different cattle farms were analyzed. As many as 186 Acinetobacter isolates were obtained: A. baumannii (6), A. courvalinii/A. vivianii (2), A. gandensis (17), A. haemolyticus (1), A. indicus (26), A. pseudolwoffii (32), A. variabilis (13), Taxon 36 (9), genomic sp. 15/16 (1) and 79 unidentified isolates. Sixteen unidentified isolates formed a distinct and cohesive MALDI-TOF MS cluster, possibly representing a novel species.

Conclusion: We have applied an effective approach based on MALDI-TOF MS, which enables quick screening of multiple Acinetobacter isolates to assess their taxonomic diversity and to identify putative novel species.

* Korespondující autor: violetta.shestivska@szu.cz


  1. Nemec A. Acinetobacter. In: Trujillo ME, Dedysh S, DeVos P, Hedlund B, Kämpfer P, Rainey FA, Whitman WB, editors. Bergey's Manual of Systematics of Archaea and Bacteria. Wiley. 2022. doi: 10.1002/9781118960608.gbm01203.pub2
  2. Dijkshoorn L, Nemec A, Seifert H. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii." Nature Reviews Microbiology 2007; 5(12): 939-951.


Supported by a grant from the Czech Science Foundation (22-05373S).

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