Česká společnost pro hmotnostní spektrometrii

(13. ročník České konference hmotnostní spektrometrie a 11. Neformální proteomické setkání - ThO-10)
Proteomics of human breast milk

Peter Konik ^1 *, Martin Selinger ¹, Milan Houška ², Eliška Kováříková ², Ladislav Čurda ³, Jiří Dušek ⁴

1. Jihočeská univerzita v Českých Budějovicích
2. Výzkumný ústav potravinářský Praha, v. v. i
3. Vysoká škola chemicko-technologická v Praze
4. Nemocnice České Budějovice, a.s

Abstrakt

Breast milk is a complex mixture of proteins, fats, carbohydrates, minerals, vitamins, ions and hormones produced by the mammary glands of mammals, including humans. The dominant protein of breast milk is casein, the main “nutritional” protein; less abundant are alpha lactalbumin that regulates lactose production; lactoferin, an iron transporter; lysosyme, an antibacterial enzyme; and secretory immunoglobulins, aiding the newborn’s immunity. In the early stages of development, it is the sole source of nutrients for the newborn. In cases, where mother’s milk is not available, such as pre-term births, there is a need for breast milk supplementation or usage of donated milk from other mothers. Donated milk can be stored frozen for up to 3 months, however, milk quality, stability and sterility is a concern. In this study, we investigated protein composition of breast milk that has been sterilised by heat (pasteurisation) or pressure (pascalisation). The preliminary results show that there are no significant differences between the sterilisation methods regarding protein content and quantity, however, they suggest some changes in structure in some less abundant proteins.

* Korespondující autor: konik@prf.jcu.cz