Česká společnost pro hmotnostní spektrometrii

(13. ročník České konference hmotnostní spektrometrie a 11. Neformální proteomické setkání - ThS-13)
Bioaccumulation of pharmaceuticals in herbivorous fish: The role of aquatic plants as a dietary source

Helena Hinterholzová ^1 *, Martin Bláha ¹, Tomáš Randák ¹, Roman Grabic ¹, Kateřina Grabicová ¹

1. Jihočeská univerzita v Českých Budějovicích, Fakulta rybářství a ochrany vod

Abstrakt

Wastewater treatment plants do not completely remove micropollutants such as pharmaceuticals; therefore, these substances are commonly found in surface waters, where they can persist and bioaccumulate in aquatic organisms. While bioaccumulation in predatory and omnivorous fish is relatively well described in the literature, there are only a very limited number of studies on herbivorous fish. However, these species may represent a key pathway for the transfer of contaminants from plants to higher trophic levels. The aim of this study was to evaluate the bioaccumulation of pharmaceuticals in the tissues of grass carp (Ctenopharyngodon idella) exposed for six months in the Čežárka biological pond (Vodňany, Czech Republic), supplied exclusively with treated wastewater. Water samples, extracts from passive samplers, grass carp tissue samples, and plant samples were analyzed using liquid chromatography with mass spectrometry detection. Of the total of 78 pharmaceuticals analyzed, ten pharmaceuticals were detected in the tissues of grass carp, most commonly antidepressants and cardiovascular drugs. The highest concentrations were found in the kidneys (the sum 33 ng/g ww), followed by the brain, liver, and plasma, while no pharmaceuticals were detected in muscles. The highest bioaccumulation factors (BAF) were observed for norsertraline (up to 5600 L/kg in the brain and 4500 L/kg in the kidneys) and sertraline (1500 L/kg in the kidneys). Pharmaceuticals found in the tissues of the grass carp were also found in aquatic plants, confirming their role as a significant reservoir and dietary source of exposure.

* Korespondující autor: hinterholzova@jcu.cz

Poděkování:

This study was financially supported by the Czech Science Foundation, grant No. GACR 25-17470S.