Česká společnost pro hmotnostní spektrometrii

(5. konference České společnosti pro hmotnostní spektrometrii - ThO-010)
Protein profile of seminal plasma and functionality of spermatozoa over the course of reproductive season in carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)

Anna Kolešová ^1 *, Petr Kotas ², Jan Štěrba ^2,3, Marek Rodina ¹, Jacky Cosson ¹, Otomar Linhart ¹

1. FROV JCU
2. 2University of South Bohemia in České Budějovice, Faculty of Science, Department of Chemistry and Bi
3. 3Institute of Parasitology, Biology Centre of Academy of Sciences of the Czech Republic

Abstrakt

The purpose of the present study was to analyze changes of seminal plasma compositions, sperm production characteristics and sperm motility traits during spawning season in rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio). The highest average percentage of motile sperm cells (95.5 ± 5.64 %) and spermatozoa velocity (265.78 ± 8.1 µm/s) was observed in mid phase of spawning at 5 s post activation in both analyzed species. Sperm volume and spermatozoa concentration generally increased first, then decreased significantly towards the end of the season in both species. Seminal plasma osmolality was 262-270 mOsmol kg-1 in carp and 232-248 mOsmol kg-1 in rainbow trout, with small variation between each phase of spawning. Protein concentration of seminal plasma was stable during the entire reproductive season in carp, however in rainbow trout the highest amount of proteins (2.14 ± 0.28 mg ml-1) was observed in the middle period of breeding. Seminal plasma composition in both experimental species was analyzed using 2D-PAGE combined with MALDI-TOF/TOF MS. 16 differentially expressed spots from rainbow trout seminal plasma and 13 protein spots from seminal plasma of carp were changed over the course of reproductive period. The proteins identified as unique are involved in the regulation of sperm motility, spermatogenesis, maintenance of sperm membrane lipid stability and antioxidant protection. The current data set on physiological and functional sperm parameters and protein patterns of seminal plasma during different time of spawning will lead to a better understanding of fertilization and gamete quality and can increase success of artificial insemination methods in these species.

* Korespondující autor: annucha1987@gmail.com

Poděkování:

The study was financially supported by the Ministry of Education, Youth and Sports of the Czech Republic - projects „CENAKVA“ (No. CZ.1.05/2.1.00/01.0024), “CENAKVA II“ (No. LO1205 under the NPU I program), Postdok BIOGLOBE (CZ. 1.07/2.3.00/30.0032), COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE) and by the Czech Science Foundation (15-03044S). Access to instruments and other facilities was supported by the Czech research infrastructure for systems biology C4SYS (project no LM2015055).