Česká společnost pro hmotnostní spektrometrii

(CEEPC/IPM/CMSC 2022 - ThP-23)
Phospho-proteomic Analysis to Explore the Microbe Associated Molecular Patterns (MAMPs) in Plant Immunity

Jianan Lu ¹, Cleidiane Zampronio ¹, Andrew Bottrill ¹, Vardis Ntoukakis ¹, Alex Jones ^1 *

1. University of Warwick

Abstrakt

Due to the climate change and agrochemicals, many important food crops have been suffering with serious challenges from various pests and diseases. The first layer in plant immunity to detect highly conserved components of microbes, such as flagellin and chitin, are called Microbe-Associated Molecular Patterns (MAMPs). These MAMP signalling pathways have been widely studied in the model plant Arabidopsis thaliana, but the overlap and differences among these mechanisms remain unclear in crop plants such as Brassica, Maize and Tomato. Phosphorylation is an excellent post-translational modification to focus on and could be identified by high-throughput Mass spectrometry (Orbitrap and timsToF pro). This project has applied MS-based phosphoproteomics to quantify the phosphorylation events in the plant immunity signalling, and it helped systematically map the conserved functional pathways and accurately identify orthologs among different plants. The mega-data will make great contributions to ‘Precision Agriculture’ which is an innovative technology and allows breeders to target specific genes and accelerate the breeding speed.

* Korespondující autor: Alex.Jones@warwick.ac.uk

Literatura

1. Couto, D. and C. Zipfel, 2016. Regulation of pattern recognition receptor signalling in plants. Nat Rev Immunol 16(9): p. 537-52.
2. Schwessinger, B. and P.C. Ronald, 2012. Plant innate immunity: perception of conserved microbial signatures. Annu Rev Plant Biol 63: p. 451-82.
3. Bigeard, J., J. Colcombet, and H. Hirt, 2015. Signaling mechanisms in pattern-triggered immunity (PTI). Mol Plant, 8(4): p. 521-39.