CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - FrO-06)
In a flash of light: Native mass spectrometry for single particle imaging with X ray free electron lasers

Alan Kádek 1,2 *, Thomas Kierspel 2,3, Kristina Lorenzen 4, Charlotte Uetrecht 2,5

  1. BIOCEV - Mikrobiologický ústav AVČR
  2. Leibniz Institute of Virology / European XFEL
  3. DESY - Deutsches Elektronen-Synchrotron
  4. European XFEL
  5. Centre for Structural Systems Biology / University of Siegen

Abstrakt

Native mass spectrometry enables the ionization and transfer of intact non-covalent protein complexes into the gas phase. As such, it is a great tool to study protein assemblies in a mass and conformation specific manner, probing structural transitions they undergo. Such transient states are highly interesting for structural biology, but generally cannot be purified and are very hard to access for complementary methods such as crystallography or electron microscopy.

Despite its sensitivity and selectivity, the structural resolution in native MS alone is limited. The amount of structural information could be vastly increased by its combination with powerful hard X ray free electron lasers (XFELs) such as the LCLS II in Stanford (USA) or the European XFEL, the world’s brightest light source in Hamburg (Germany). One of the science drivers for these facilities has been enabling structural studies on single particles. However, these efforts have been hindered by the lack of efficient ways to introduce samples into the X-ray beam. [1] In this regard, native MS offers huge benefits in producing and transmitting molecular ions ranging in size from single proteins and non-covalent protein complexes up to multi-megadalton viral particles. Also, profiting from ion charges, MS can select and separate protein complexes based on their mass and conformation and possibly also pre-orient particles to simplify subsequent data processing and structure determination. [2,3]

This contribution will present the work and current development status of the highly interdisciplinary MS SPIDOC consortium, which works toward a native MS-based sample introduction system for use at beamlines of the European XFEL and other light sources.

* Korespondující autor: alan.kadek@biomed.cas.cz

Literatura

  1. J. Bielecki, F. Maia, A. Mancuso: Struct. Dyn. 7, 040901 (2020)
  2. A. Kadek, K. Lorenzen, C. Uetrecht: Drug Discov. Today Technol. 39, 89–99 (2021)
  3. E. Marklund, T. Ekeberg, M. Moog, J. Benesch, C. Caleman: J. Phys. Chem. Lett. 8, 4540–4 (2017)

Partneři společnosti

LabRules LCMS LabRules GCMS

Partneři

Bruker HPST Merck Pragolab Amedis EastPort Shimadzu Waters