CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - ThP-21)
The Omics Hunt for Novel Molecular Markers of Resistance to Phytophthora infestans

Miroslav Berka 1 *, Marie Greplová 2, Šarlota Shejbalová 1, Břetislav Brzobohatý 1, Martin Černý 1

  1. Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno
  2. Potato Research Institute, Ltd., 58001, Havlíckuv Brod

Abstrakt

Wild Solanum accessions are a treasured source of resistance against pathogens, including oomycete Phytophthora infestans, causing late blight disease. We analyzed
Solanum pinnatisectum, Solanum tuberosum, and the somatic hybrid between these two lines, representing resistant, susceptible, and moderately resistant genotypes,
respectively. Proteome and metabolome analyses showed that the infection had the highest impact on leaves of the resistant plant and indicated, among others, an
extensive remodeling of the leaf lipidome. The lipidome profiling confirmed an accumulation of glycerolipids, a depletion in the total pool of glycerophospholipids, and
showed considerable differences between the lipidome composition of resistant and susceptible genotypes. The analysis of putative resistance markers pinpointed more
than 100 molecules that positively correlated with resistance. Putative resistance protein markers were targeted in an additional 12 genotypes with contrasting resistance
to P. infestans. At least 27 proteins showed a negative correlation with the susceptibility including HSP70­-2, endochitinase B, WPP domain-­containing protein, and
cyclase 3. In summary, these findings provide insights into molecular mechanisms of resistance against P. infestans and present novel targets for selective breeding.

* Korespondující autor: miroslavberka94@gmail.com

Poděkování:

This research was funded by the project NAZV QK1910045 “Identification of Metabolites Correlating with Quantitative Resistance to Phytophthora infestans”.


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Partneři

Bruker HPST Merck Pragolab Amedis EastPort Shimadzu Waters