Česká společnost pro hmotnostní spektrometrii

(13. ročník České konference hmotnostní spektrometrie a 11. Neformální proteomické setkání - ThO-12)
DECIPHERING ADAPTIVE SHIFTS IN THE REDOX PROTEOME OF WHEAT SEEDLINGS UNDER DROUGHT

Olha Lakhneko ^1,2, Mohammad Umar ², Oleg Stasik ³, Lenka Kohútová ⁴, Maksym Danchenko ^2 *

1. Institute of Cell Biology and Genetic Engineering, NASU, Kyiv, Ukraine
2. Plant Science Biodiversity Centre, SAS, Nitra, Slovakia
3. Institute of Plant Physiology and Genetics, NASU, Kyiv, Ukraine
4. Institute of Chemistry, SAS, Bratislava, Slovakia

Abstrakt

Global warming increases the frequency of extreme drought events. Limited water availability for crops negatively affects yields in many regions of the planet, including Europe, thereby compromising food security. Bread wheat (<i>Triticum aestivum</i>) is vital for human nutrition. Exploring responses of contrasting plant genotypes enables a complex understanding of phenotypes challenged by drought and, eventually, facilitates the design of resilient crops.
Drought affects crops on molecular, biochemical, physiological, and morphological levels. Moreover, plant responses to water shortage stress are frequently associated with posttranslational modifications (PTMs) of versatile proteins. Such modifications, including reversible oxidation of cysteine thiols (redox), regulate protein functions: Activity, stability, subcellular localization, and signal perception/transduction. Redox proteome dynamics in plant leaves under stress conditions is a novel emerging field of study.
The redox state of protein cysteines regulates plant resilience during water shortage and recovery. We explore the variable impact of water deprivation and recovery on the redox proteome dynamics in leaves of wheat cultivars contrasting in drought tolerance using resin-assisted capture. The study started elucidating drought- and recovery-responsive components of the wheat redox proteome, yielding candidate markers and pathways of resilience, supported by the spatial distribution of reactive oxygen species (ROS) and expression dynamics of redox-regulating genes, thereby linking biochemical, cellular, and molecular changes. New knowledge will facilitate maintaining a stable yield under an extreme climate.

* Korespondující autor: maksym.danchenko@savba.sk

Poděkování:

This study was supported by the Slovak Research and Development Agency grant APVV-23-0407 and the Slovak Academy of Sciences project National Academy of Sciences project VEGA 2/0106/22.