CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - FrP-14)
Phytophthora resistance mechanisms in diverse plant species

Miroslav Berka 1, Iñigo Saiz-Fernández 1, Ivan Milenković 1, Jaroslav Ďurkovič 2, Miloň Dvořák 1, Břetislav Brzobohatý 1, Martin Černý 1 *

  1. Mendel University in Brno
  2. Technical University in Zvolen

Abstrakt

Poplars are among the fastest-growing trees and significant resources in agriculture and forestry. However, rapid growth requires a large water consumption, and irrigation water provides a natural means for pathogen spread. That includes members of Phytophthora spp. that have proven to be a global enemy to forests. Here, the effects of artificial inoculation with two different representatives of aggressive species (P. cactorum and P. plurivora) were analyzed in the proteome of the Phytophthora-tolerant hybrid poplar clone T-14. Wood microcore samples were collected at the active necrosis borders to provide insight into the molecular processes underlying the observed tolerance to Phytophthora. The analysis revealed the pathogen impact on poplar primary and secondary metabolism, including carbohydrate-active enzymes, amino acid biosynthesis, phenolic metabolism, and lipid metabolism, all of which were confirmed by consecutive metabolome and lipidome profiling. The identified Phytophthora response proteins were compared to those previously found in trees with compromised defense against Phytophthora, namely, Quercus spp. and Castanea sativa. That provided a subset of candidate markers of Phytophthora tolerance, including certain ribosomal proteins, auxin metabolism enzymes, dioxygenases, polyphenol oxidases, trehalose-phosphate synthase, mannose-1-phosphate guanylyltransferase, and rhamnose biosynthetic enzymes. In summary, this analysis provided the first insight into the molecular mechanisms of hybrid poplar defense against Phytophthora and identified prospective targets for improving Phytophthora tolerance in trees.

* Korespondující autor: martincerny83@gmail.com

Poděkování:

This work was supported by the European Regional Development Fund, Project Phytophthora Research Centre Reg. No. CZ.02.1.01/0.0/0.0/15_003/0000453 and by funding from the Slovak scientific grant agency VEGA (1/0450/19 and 1/0108/23).


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Partneři

Bruker HPST Merck Pragolab Amedis EastPort Shimadzu Waters