CEEPC/IPM/CMSC - Abstrakt prezentace

(Česká konference hmotnostní spektrometrie 2017 - P-010)
MALDI-TOF MS profiling as a tool for identification of sand fly juvenile stages

Kristýna Hlavačková 1, Renata Ptáčková 2,1, Vít Dvořák 1, Petr Volf 1, Petr Halada 2 *

  1. Přírodovědecká fakulta, Univerzita Karlova v Praze
  2. Mikrobiologický ústav AV ČR, v.v.i.

Abstrakt

Phlebotomine sand flies are small blood-sucking insects of high medical and veterinary significance. Females represent well-known vectors of viruses, bacteria, and most importantly parasites of the genus Leishmania causing leishmaniasis. To ensure proper vector control and monitoring in epidemiological studies a method for accurate species identification is highly desirable. Recently, we have introduced MALDI-TOF protein profiling as a reliable approach for species differentiation of adult sand flies. Our ongoing research is further focused on sand fly immature stages precise identification of which is practically impossible using classical morphological approaches.
The aim of the study was to evaluate the usefulness of MALDI-MS for identification of different life stages of sand flies and map the changes in their protein profiles during the whole life cycle. Specimens of six different sand fly species from the L2 to L4 larval stages and pupal stage were subjected to MALDI-TOF analysis. For larvae, the spectra were reproducible and easily distinguishable at the species level, and the profiles were stable from the L2 to L4 developmental stages. Therefore only profiles of L4 stages were selected for the creation of juvenile reference database. The performance of the database was tested in a blind study where 123 specimens (from L2 to L4) of six different species were included. The test resulted in 92.7% correct identifications regardless the larval stage. Pupal spectra allow identification at species level as well and surprisingly, they did not change during metamorphosis as observed for other holometabolous insects. The study demonstrates that MALDI-TOF MS is a suitable method for identifying not only adults, but also immature stages of phlebotomine sand flies.

* Korespondující autor: halada@biomed.cas.cz

Literatura

  1. Dvorak V. et al.: Parasit. Vectors 7, 21 (2014).

Poděkování:

The financial support from Grant Agency of the Czech Republic (15-04329S) and from Institute of Microbiology (RVO61388971) is gratefully acknowledged.


Partneři společnosti

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Partneři

Bruker HPST Merck Pragolab Amedis EastPort Shimadzu Waters