CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - FrP-04)
Determination of animal origin of recent and archaeological bones by mass spectrometry

Alena Meledina 1 *

  1. VŠCHT


The skeletal remains of vertebrates or more often their fragments are among the most common findings at archaeological sites. The determination of the animal origin of the bones is therefore necessary in the field of archeology and paleontology, as well as in the field of food safety (control of compliance with marketing standards for meat-bone meal) and forensic analysis.
The aim of this work is to develop a new proteomic methodology suitable for the differentiation of animal origin of bones based on their protein composition.
This work is based on proteomic analysis of bone samples using two mass spectrometry (MS) techniques – MALDI–TOF (Matrix–Assisted Laser Desorption/Ionization – Time of Flight) and LC–ESI–Q–TOF (Liquid Chromatography – Electrospray Ionization – Quadrupole – Time of Flight). The mass spectra obtained from MALDI–TOF MS were searched for unique markers in the form of m/z values for each species. Characteristic markers represent a reliable tool enabling a rapid identification of the animal origin of bone samples. Due to the data from LC–ESI–Q–TOF, specific proteins and their peptide fragments were identified in the form of amino acid sequences, from which specific markers for each animal were selected.
The obtained results demonstrate that using these two MS techniques, the animal origin of the bone samples can be determined based on the differences in their protein composition. This information can be used to determine the animal species which the found archaeological bone material comes from. Based on the found characteristic markers, it is possible to create a database that will enable the identification of the animal origin of unknown samples faster and more reliably than currently used techniques (e.g. DNA analysis).

* Korespondující autor: meledina@vscht.cz


This work was supported from the grant of Specific university research – grant No. A2_FPBT_2022_042 and A2_FPBT_2022_001.

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