CEEPC/IPM/CMSC - Abstrakt prezentace

(5. konference České společnosti pro hmotnostní spektrometrii - ThO-011)
Advanced glycomic profiling in cancer research

Milos V. Novotny 1,2 *

  1. Indiana University, Bloomington, Indiana, USA
  2. RECAMO, Masaryk Memorial Onclogical Institute, Brno, CZ

Abstrakt

Both N- and O-linked glycans often appear as structural determinants in the most important biological interactions involving immunity and cancer. Quantitative profiling of glycans in physiological fluids and biopsies from cancer patients can thus provide a wealth of clinically useful information. Different analytical methods have been employed in acquisition of glycan profiles in different types of cancer and its stages: mostly MALDI- and ESI-mass spectrometry (MS), but also HPLC-fluorescence and microchip electrophoresis/laser-induced fluorescence (LIF) detection. Analytical outputs from different patient cohorts must then be rigorously evaluated through the clinically established statistical procedures. Biochemical interpretation of glycosylation mechanisms necessitates exact structural assignments, which are not always clear from typical analytical data. Cancer biomarker search often implicates the needs to resolve glycan isomers as a part of such interpretations. The currently explored approaches to isomeric resolution of oligosaccharides involve ultrahigh-pressure capillary LC, microchip electrophoresis, or ion-mobility spectrometry used prior to mass spectrometry. Glycomic profiling applications will be exemplified through the cancer samples of blood serum, ascitic fluids and urinary exosomes. During recent investigations, emphasis has been placed on certain classes of N-glycans featuring different positions of fucosyl residues, bisected structures, α-2,3- vs. α-2,6-linked sialyl-substituted multiantennary glycans, and paucimannosidic structures. It can be shown that the MS-based profiling and microchip electrophoresis/LIF provide complementary analytical data. However, O-glycan profiling necessitates alternative sample treatment and analytical strategies.

* Korespondující autor: novotny@indiana.edu


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