CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - ThP-03)
LC-MS/MS Analysis of Short-Chain Fatty Acids and Their Derivatives in the Plasma of Women with Gestational Diabetes Mellitus

Eliška Ivanovová 1, Barbora Piskláková 1, Jaroslava Friedecká 1, Ondřej Krystyník 1, David Friedecký 1, David Karásek 1 *

  1. University Hospital Olomouc and Faculty of Medicine and Dentistry, Palacký University Olomouc

Abstrakt

Gestational diabetes mellitus (GDM) represents a heterogeneous group of hyperglycemic metabolic disorders. Currently, the diagnosis of GDM is based on repeated measurements of elevated fasting plasma glucose (FPG) or on results showing elevated postprandial plasma glucose (PPG). However, recent research has focused on studying changes in the gut microbiome of women during pregnancy. Metabolic changes may cause gut dysbiosis in pregnant women and thus the development of GDM. The products of gut bacteria, short-chain fatty acids (SCFA) and their derivatives, may play a vital role in the diagnosis of GDM. Current studies use the classification of women with GDM mainly according to the week/trimester of pregnancy. However, this study compares groups of women with GDM according to the method of diagnosis of the disease. For the analysis of SCFA and their derivatives, the LC-MS/MS method was optimized according to the validated protocols by Jaochico et al. (2019) and Shafaei et al. (2021). SCFA and their derivatives were derivatized and separated by reversed-phase liquid chromatography (RPLC) and detected by triple quadrupole mass spectrometer (QqQ). Fatty acids were evaluated along with baseline body composition characteristics and biochemical parameters in women with three different GDM phenotypes, healthy pregnant and non-pregnant women. 3-hydroxybutyrate (3-OH-BA), 4-methylvalerate (4-MVA) and isovalerate (IVA) were assessed as statistically significant within the groups of women measured. 3-OH-BA was elevated in all three groups of women with different GDM phenotypes. Increased/decreased concentrations of 4 MVA/IVA were found in all groups of pregnant women. In comparison with previously published studies, we found new potential biomarkers for GDM, 4-MVA and IVA.

* Korespondující autor: david.karasek@fnol.cz

Poděkování:

This project was supported by the Ministry of Health of the Czech Republic (NV18-01-00139, NU20-08-00367), Doctoral Study Grant of Palacký University Olomouc (DSGC-2021-0098) and FNOl (00098892).


Partneři společnosti

LabRules LCMS LabRules GCMS

Partneři

Bruker HPST Merck Pragolab Amedis EastPort Shimadzu Waters