CEEPC/IPM/CMSC - Abstrakt prezentace

(CEEPC/IPM/CMSC 2022 - FrP-02)
Development of on-line pretreatment methods for growth factor analysis using CZE-MS

Martina Opetová 1 *, Radovan Tomašovský 1, Peter Mikuš 1,2, Katarína Maráková 1,2

  1. Faculty of Pharmacy, Comenius University in Bratislava
  2. Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava

Abstrakt

Growth factors are a heterogeneous group of proteins that are secreted by various tissues in the body. Their presence in biological samples may indicate the development of various diseases, so there is a growing need to develop new high-performance analytical methods and procedures for their identification and quantification. The developed methods could subsequently be used either in medicine and diagnostics, or in therapeutic monitoring and anti-doping. The aim of this work is the development of on-line pretreatment methods aimed at concentrating the sample before the analysis itself using an on-line combination of capillary zone electrophoresis (CZE) and mass spectrometry (MS) [1,2]. Optimization and comparison of two pretreatment methods, transient isotachophoresis (tITP) and dynamic pH junction (DPJ), was performed on a mixture of standards of three selected intact growth factors, namely IGF-1 (insulin-like growth factor-1), EGF (epidermal growth factor), and TGF-α (transforming growth factor- α). The lowest detection limits of 66 ng/mL for IGF-1 and EGF and 40 ng/mL for TGF- α were reached using the transient isotachophoresis (tITP) method and using 200 mmol/L ammonium formate (pH 4.0) as the leading electrolyte. The benefit of such a method is a prerequisite for its use in the analysis of growth factors as potential biomarkers of diseases in biological samples, where these substances are found at very low concentration levels.

* Korespondující autor: opetova2@uniba.sk

Literatura

  1. Wu, H. et al: Reviews in Analytical Chemistry 39(1), 45-55 (2020).
  2. Nickerson, J. L. et al: Mass Spectrometry Reviews (2021).

Poděkování:

This work was supported by the grants VEGA 1/0483/20, VEGA 1/0514/22, UK/93/2022, and UK/110/2022 and carried out in the Toxicological and Antidoping Center at the Faculty of Pharmacy, Comenius University in Bratislava.


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